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VIID III Microbiology Mid-Semester Exam 2025 Questions and Answers What are Koch's postulates for a pathogenic organism? (4 marks) - ....ANSWER ...- - The agent must be present in every case of the disease - The agent must be isolated and cultured in vitro - The disease must be reproduced when a pure culture of the agent is inoculated into a susceptible host - The agent must be recoverable from the experimentally-infected host Explain Robert Koch's contribution to bacteriology: - ....ANSWER ...-Pure culture technique, isolation of cause of anthrax, cause of tuberculosis, cause of cholera, proposed Koch's Postulates "Father of infectious diseases" Explain Louie Pasteur's contribution to bacteriology: - ....ANSWER ...-Debunked Spontaneous Generation Theory (Germ Theory), basis for fermentation, pasteurisation, brewing, vaccination, contagious disease (rabies, anthrax, fowl cholera) ← discovered the causes Explain Joseph Lister's contribution to bacteriology: - ....ANSWER ...-Concept of antiseptics (carbolic acid), Aseptic technique for surgical procedures (sterilisation w. Pasteur), souring of milk (first isolation of bacteria) …FOR STUDENTS ONLY…©️2025 ALL RIGHTS RESERVED… 1