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IUPUI Cell Biology Exam #2 2025 Questions and Answers cDNA library - ....ANSWER☑️☑️...-Collection of DNA fragments synthesized using all of the mRNAs present in a particular type of cell as a template. complementary DNA (cDNA) - ....ANSWER☑️☑️...-DNA molecule synthesized from an mRNA molecule and therefore lacking the introns that are present in genomic DNA. dideoxy sequencing or Sanger sequencing - ....ANSWER☑️☑️...-The standard method of determining the nucleotide sequence of DNA; utilizes DNA polymerase and a set of chain-terminating nucleotides. DNA cloning - ....ANSWER☑️☑️...-Production of many identical copies of a DNA sequence; can be made by forcing DNA to copy sequences DNA library - ....ANSWER☑️☑️...-Collection of cloned DNA molecules, representing either an entire genome (genomic library) or copies of the mRNA produced by a cell (cDNA library). DNA microarray - ....ANSWER☑️☑️...-cDNA are made from samples and labeled with fluorescent dyes; expression of different mRNAs of each sample is monitored by examining the intensity and color of the fluorescent signals gene knockout - ....ANSWER☑️☑️...-A genetically engineered animal in which a specific gene has been inactivated. …FOR STUDY PURPOSES ONLY…©️ 2025 ALL RIGHTS RESERVED… 1